John A. Conrad, Ph.D.
- Associate Professor
- 349 DSC
- General Chemistry, Biochemistry
Additional Information
Research Interests
My research involves using absorbance spectroscopy to study the mechanistic details of flavoproteins involved in the biodegradation of aromatic compounds.
Publications
Conrad, J.A., *Hoppe, S.W., Ortiz-Maldonado, M.; Palfey, B.A. Detection of intermediates in the oxidative half-reaction of the FAD-dependent thymidylate synthase from Thermotoga maritima: carbon transfer without covalent pyrimidine activation. Biochemistry, 2014, 53, 32.
Youngblut, M.; Pauly, D.J.; Stein, N.; Walters, D.; Conrad, J.A.; Moran, G.R.; Bennett, B.; Pacheco, A.A. Shewanella oneidensis cytochrome c nitrate reductase (ccNir) does not disproportionate hydroxylamine to ammonia and nitrate, despite a strongly favorable driving force. Biochemistry, 2014, 53, 13.
Shah, D.D.; Conrad, J.A; Moran, G.R. Intermediate partitioning kinetic isotope effects for the NIH shift of 4-hydroxyphenylpyruvate dioxygenase and the hydroxylation reaction of hydroxymandelate synthase reveal mechanistic complexity. Biochemistry. 2013, 52, 35.
Michanina, T.; Kohen, E.M.; Conrad, J.A.; Palfey, B.A.; Lesley, S.A.; Kohen, A. Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase. J.A.C.S., 2012, 134, 9.
Shah, D.D.; Conrad, J.A.; Heinz, B.; Brownlee, J.M.; Moran, G.R. Evidence for the mechanism of hydroxylation by 4-hydroxyphenylpyruvate dioxygenase and hydroxymandelate synthase from intermediate partitioning in active site variants. Biochemistry, 2011, 49, 9.
Additional Information
Research Interests
My research involves using absorbance spectroscopy to study the mechanistic details of flavoproteins involved in the biodegradation of aromatic compounds.
Publications
Conrad, J.A., *Hoppe, S.W., Ortiz-Maldonado, M.; Palfey, B.A. Detection of intermediates in the oxidative half-reaction of the FAD-dependent thymidylate synthase from Thermotoga maritima: carbon transfer without covalent pyrimidine activation. Biochemistry, 2014, 53, 32.
Youngblut, M.; Pauly, D.J.; Stein, N.; Walters, D.; Conrad, J.A.; Moran, G.R.; Bennett, B.; Pacheco, A.A. Shewanella oneidensis cytochrome c nitrate reductase (ccNir) does not disproportionate hydroxylamine to ammonia and nitrate, despite a strongly favorable driving force. Biochemistry, 2014, 53, 13.
Shah, D.D.; Conrad, J.A; Moran, G.R. Intermediate partitioning kinetic isotope effects for the NIH shift of 4-hydroxyphenylpyruvate dioxygenase and the hydroxylation reaction of hydroxymandelate synthase reveal mechanistic complexity. Biochemistry. 2013, 52, 35.
Michanina, T.; Kohen, E.M.; Conrad, J.A.; Palfey, B.A.; Lesley, S.A.; Kohen, A. Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase. J.A.C.S., 2012, 134, 9.
Shah, D.D.; Conrad, J.A.; Heinz, B.; Brownlee, J.M.; Moran, G.R. Evidence for the mechanism of hydroxylation by 4-hydroxyphenylpyruvate dioxygenase and hydroxymandelate synthase from intermediate partitioning in active site variants. Biochemistry, 2011, 49, 9.